Comparing Specimens to Detect C. trachomatis and N. gonorrhoeae

Comparing Specimens to Detect C. trachomatis and N. gonorrhoeaeChlamydia trachomatis can commonly found to sexually active females.

That is why it is recommended for them by health policy organization guidelines to have a regular chlamydial screening. First method to detect C. trachomatis is the use of endocervical samples to Nucleic acid amplification technique (NAAT), it has found have sensitivities range from 75-100%, and mostly of the report has found to have greater than 90%.

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Another method is the use of first-void urine (FVU) samples to NAAT, it has found to have 50-95% sensitivities. And the last method is the use of self-collected vaginal samples to NAAT, it is also found to have 75-100% success rate to detect sexually transmitted (STD) specimens from young adult women. When it comes to Neisseria gonorrhoeae, the performance of NAAT is found to be similar to those C. trachomatis. Endocervical sample’s ability to detect is found to be 89-97%, FVU sample has 65-93%, and vaginal sample has greater than 90% result.Performance profiles of NAATs vary depending on the choice of STD test, type of specimen, and the population studied. There is a study conducted in Western Australia wherein it is found out that self-collected vaginal swab method gives more positive tests for C. trachomatis and N.

gonorrhoeae through polymerase chain reaction (PRC). Therefore, this study is done to find capability of different types of specimen, as used to ligase chain reaction assay, to detect C. trachomatis and N. gonorrhoeae using.The subject of the study includes 1,841 young women entering to the U.S. Marine Corps Recruiting Depot. They are given an examination period which includes giving them a self-administered questionnaire and asking for specimens.

They conducted three types of specimen collection; these are endocervical, FVU, and self-collected vaginal swab.Among the three types of specimen collection for C. trachomatis self-collected vaginal swab got the highest ability to detect a positive result, which is 81%. The next best type is FVU which got 72%, and the last is endocervical specimen with 64%. And the combination of FVU and vaginal specimen got the highest result with 94%. It is expected that combinations of tests will outperformed any single test.For N.

gonorrhoeae, the ability to detect it is poorer in all single test compare with C. trachomatis. For a single test, vaginal specimen still got highest detection with 72%, followed by endocervical specimen with 49%, and the last is FVU with 24%. For the combinations of test, endocervical and vaginal specimen got the highest detection with 93%.

Having self-collected vaginal specimen highest detection have also been discovered from other studies conducted before as well as the result that combinations of tests will produce higher detection as compare with single tests. However, having endocervical got the lowest detection differ from the studies conducted before.Since the epidemic is spreading among women, it is necessary to increase screening. The study is significant to the development of consumer-friendly STD screening test, those that are acceptable, accurate, stable, and widely available to women. And it is recommended to have further studies for this issue to have more ideal screening specimen.This study is chosen because this is very informative and gives awareness about STD. Also, it gives women more knowledge about their body and how to properly care for it.

The presentation is very detailed and it gives the reader more data in order to understand the study. However, it is very technical for a normal people to read and is very difficult to understand at first reading. Some research regarding some terms is needed to able to understand the paper.ReferencesShafer, M.

, Moncada, J., Boyer, C., Betsinger, K., Flinn, S., Schachter, J. (2003). Comparing first-void urine specimens, self-collected vaginal swabs, and endocervical specimens to detect Chlamydia trachomatis and Neisseria gonorrhoeae by a nucleic acid amplification test.

Journal of Clinical Microbiology, 41, p.4395-4399.