In and SOD3 were significantly influenced by irrigation

In this research, three isoforms for SOD and only one isoform for CAT were
detected. Figures 2 and 3 are examples for banding patterns of SOD and CAT
enzymes in borage from primed and unprimed seeds under different irrigation
intervalsResults of analysis of variance showed that relative activities of SOD1
and SOD3 were significantly influenced by irrigation treatments (p
? 0.01), but the effect of seed priming on these isoforms were not significant
(p > 0.05). In contract, water deficit and also seed priming had no
significant effect on SOD2 activity (p > 0.05). The
interaction of irrigation × priming was not significant for SOD isoforms (p
> 0.05). A decrease was observed in SOD1 and SOD3
activities under mild water deficit (I2) in comparison with the well
watering (14% and 8.1%, respectively). However, relative activity of SOD1
and SOD3 were severely increased with reducing water availability.
The activity of SOD1 was 41.5% and 29.1% higher in the plants
irrigated with intervals of 120 mm (I3) and 150 mm evaporation (I4)
compared to well watering, respectively. Moderate (I3) and severe
water deficit (I4) also led to a 52.7% and 48% increase in the
relative activity of SOD3, respectively (Figure 4). Although, SOD2
activity was not significantly changed in leaves under different levels of
irrigation (p > 0.05), moderate (I3) and severe water
deficit (I4) increased relative activity of SOD2.Relative activity of CAT was significantly affected by irrigation intervals
(p ? 0.01), but the effect of seed priming on this enzyme was not
significant (p > 0.05). CAT activity of plants from primed and
unprimed seeds was similar in response to drought stress (p > 0.05).
All plants from primed and unprimed seeds showed the highest CAT activity under
well watering (I1), but all limited irrigation treatments
significantly and almost similarly decreased relative activity of CAT, compared
with well irrigation (Figure 5).