LAB produces various antimicrobialcompounds, like: pH-reducing products lactic and acetic acids, hydrogenperoxide, formic acid, propionic acid usually, food antimicrobial agents aren’tused individually to control food infections but they are part of the multiple accessto control microbial load. Lactic acid bacteria (LAB) from fermented products arereservoir of antimicrobial-resistance genes that could be transmitted to the pathogens,either in the food web or, in the gastrointestinal tract of human and animal. Essential products of higher plants may controldifferent origin of antimicrobial agents with conceivable different mechanismsof action. They are useful in the treatment of infection while together a lotof the reactions that are often associated with antimicrobials. “Antimicrobial agent” a term used torefer to any compound which consists of antibiotics.
Antimicrobial agents are essential during the new product manufacturingprocess or existing product development process and become a center part of theproduct at the surface. When micro-organisms such as bacteria, molds come in contactwith the product surface, our antimicrobial agents goes into the cell wall ofthe micro-organisms and mess up the functions to restrict microbial growth andreproduction. Yogurtmaking was originally based on awareness and experimental processes left outcommon procedures or examination of the impression that occur during theprocess.
After the delayed 20th century, yogurt became a commercially good, itsmanufacture became industrialized and the processes were standardized. Duringthe latest 20 years, interest in yogurt making has increased exceedingly forscientific and commercial reasons. Experimental findings have recommended newdairy products that help human health (probiotic cultures, fortification withbioactive compounds) further with enhanced sensory, exclusively texturalcharacteristics. Consumer show interest for yogurt and same fermented dairyproducts has increased. Food spoilage introduce to many changes in which thefood becomes acceptable for consumption or even toxic to consumers thesechanges may be accompanied by changes in taste, smell, appearance of Tomato andit is belongs to the family solanaceae. One of the most important principles fora probiotic collection is the effectiveness to increase innate host defenses byproduction of antimicrobial products, and the growth inhibition or competitiveexclusion of the enteric pathogens.
During the test the cultures were kept inMRS agar stabs at refrigeration temperature. MRS agar plates and repeatedlystreaked on MRS agar plates and were incubated to obtain a pure culture. Alsogrowth on PDA plates was repeatedly sub-cultured to obtain pure cultures.Slants were prepared respectively in McCartney bottles, inoculated andincubated at 37°C for 24h (Bacteria) and 25°C for 3-5days (Fungi). Purecultures were stored in the refrigerator at a temperature of 4°C as stockculture.The main objective of our work is the isolation of L.plantarum and its antifungal properties.
In this we use yogurt and tomato for the isolation of L.plantarum and we also isolate different strains of fungusto observe the antifungal effect of Lactobacillus. In this we observe thecolonial morphological characteristics of other lactobacillus strains otherthan L.plantarum as well asFungal strains. I would like to conclude my work as L.plantarum is easily available in yogurt and tomato and ithas vast benefits such as its antifungal activities against different types offungi.
We can take benefit of it and can use it in different fungal infectionsas well as we can also use it as prophylaxis of different fungal infections. AsL.plantarum show zone ofinhibition against all the types of Fungi so it can be said that it is broadspectrum and can be easily isolated from normal natural routinely food materialsuch as yogurt and tomato. We must take benefit of this natural gift of nature.