Lab Report

Cells comprising rapidly growing tissues, like those in the root tip of a plant spend a comparatively long time in the mitotic phase, whereas those cells imprisoning slow-growing tissues would spend most of their lives in enterprise. Non-dividing cells remain in enterprise and never enter the mitotic phase.

(Lab Manual 64) Enterprise is the synthesis of biological molecules including DNA and duplicated DNA with associated proteins. These comprise the chromatin that begin to condense toward the end of this phase, but are not yet visible. The nucleolus’s has a uniform grainy appearance.Protease is when the chromatin condenses into the more distinct elongate strands early in protease. Also nucleoli and the nuclear membrane begin to disappear. The mitotic spindles take formation at opposing ends of the cell. The chromatin is visible, but only in threads.

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Metastases is the most distinctive of all stages of mitosis, because it is characterized by the regular arrangement of chromosomes at a central region called the metastases plate. Now Anapest beings as duplicate chromosomes separate, moving to opposite poles of the cell.Telephone is marked by the formation of a nuclear membrane around two daughter nuclei. Also chromosomes begin uncoiling and less distinct.

Cytokines begins at this stage, which is shown with the development of a cell plate in plants cells. Hypothesis: Protease will be the phase that the cells spend the most time in, because so many things are occurring as opposed to other cells. Chromatin begin to appear as rods, the disappearance of the nuclear membrane and nucleoli, the formation of spindles. A number of things occur in this phase; therefore more than a quarter of time will be spent in protease over 16 hours.Materials and Methods: This lab will examine the prepared microscope slides of the rapidly growing imperialistic tissue of the root tips of the onion Ilium. This tissue is particularly suitable for us since the tissue showing the greatest mitotic activity is quite localized. Use the illustrations provided to examine and classify the cells seen in the microscope. Begin this activity by pointing the root tip downward in the field and microscope on low power (XX-yellow objective) place the pointer at the root tip in the region just behind the root cap.

This area is where cells begin their arrangement into columns.Starting in this region proceed upward in the center of the root along a single column of cells. This process is designed to place ACH observer in about the same location on the root tip before beginning the classifying. Now without moving the slide, change the objective to high power (XX, blue objective).

With the pointer of the ocular placed in the 12 o’clock position, pointing downward, proceed down the column of cells indicated by the tip of the pointer and record the mitotic stage of the first 25 cells you encounter in that column. If you encounter cells that do not contain nuclei or it is too out of focus, continue your examination.Determine which of these alternate columns o choose, flip a coin. Record all your data in the tables. After the first 25 return to low power and start back the tip of the root.

Repeat this sequence for the second, third, and fourth data sets. Each time that you begin a new data set reset to low and move back to the onion tips meanwhile starting at a different position, for instance you started at 12 o’clock, move to the 3, the 9, and then finally the 6 o’ clock positions. Create a table of data for the entire class and make a pie chart showing percentage of time that the cells spent in each of phase of mitosis.