The resistance of bacteria and fungi are developed in the last fewyears against antibiotic and many families of antibiotic aren’t effective andthe old families of antibiotic aren’t able to treat a lot of pathogenicinfection so we must find new ways todiscover new antibiotic has powerful action against bacteria and fungi to overcome the resistance of bacteria and fungi and the best way to find new antibiotics is to discover new microorganismsable to produce new effective antibiotics . The best source of antibiotics are actinomycetes so a lot of types of antibiotics in our life and common use inour life for most pathogenic infection are from actinomycetes .Actinomycetes are still novel source of antibiotics so we have to find new sourceof actinomycetes and new microorganisms as anew source of antibiotics(Thermophelic actinomycetes ). Actinomycetes are still important source formany drugs , herbicides, insecticidesand anti-parasitic compounds particularly streptomyces spieces( Williams, 1982; Aghighiet al.
, 2004).Resistance of pathogenic bacteria and fungi are still abig problemin our life and this back to in few lastyears the antibiotics can easily control on pathogenic microorganism but nowaday it is very difficult to control the infection and this is a reason for resistanceif antibiotics . In our study we try to find rare group of thermophelicactinomycetes and study it’s microbial activity against pathogenic , (Wedberg1966 )organismsand to improve it’s production of antimicrobial substance by studingphysiological and biochemical factors affected the growth of actinomycetes andit’s antimicrobial production So the development of newer antimicrobial drugs, the polyene andnon polyene antimicrobials still one of the most potent broad spectrum antibiotics available for clinical use. As a result ofthe continuing search for new polyenes and non-polyenes, as well as thelimitations of current production methods, in our study, we isolate and characterize thermophilic Streptomycesand it’s antimicrobial activity and the improvement of production, purificationand identification of antimicrobial substanceAim of the work: 1- Isolation andcharacterization of thermophilic Streptomyces with antimicrobial activity2- Optimizing the production conditions and purification process ofthe bioactive antimicrobial agents.